Short exposures to tunicamycin induce apoptosis in SV40-transformed but not in normal human fibroblasts
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چکیده
منابع مشابه
SV40 T-antigen is required for maintenance of immortal growth in SV40-transformed human fibroblasts.
Two lines of immortal human fibroblasts were isolated following transfection of TIG-3 cells with plasmid DNA, pMT-1ODtsA, that contained SV40 early gene with a deletion in replication origin and ts mutation in coding sequence for T-antigen. These cells continued proliferation at 34 degrees C, over 565 population doubling level (PDL) which is far over the limited division potential of untransfor...
متن کاملTransformed but not normal hepatocytes express UCP2.
Uncoupling protein 2 (UCP2) expression in liver is restricted to non-parenchymal cells. By means of differential display screening between normal rat liver and H4IIE hepatoma cells we have isolated a cDNA clone encompassing part of UCP2 cDNA. Northern blot analysis revealed that UCP2 is expressed in some hepatocarcinoma cell lines, while it is absent in adult hepatocytes. UCP2 mRNA in H4IIE cel...
متن کاملAscorbate induced changes in glycosaminoglycan synthesis and distribution of normal and SV40-transformed fibroblasts.
The effect of ascorbate on the glycosaminoglycans synthesized by normal and simian virus 40(SV40)-transformed human skin fibroblasts was examined. Cells were incubated in the presence or absence of ascorbate, and radiolabelled with [3H]glucosamine and [35S]sulphate for 48 h, 3 days after reaching confluence. Glycosaminoglycans were analysed in the medium, a collagenase extract, and in the tryps...
متن کاملManganese-containing superoxide dismutase overexpression causes phenotypic reversion in SV40-transformed human lung fibroblasts.
Manganese superoxide dismutase (MnSOD) has been found to be low in a wide range of tumor cells as well as in vitro-transformed cell lines and has been implicated as a new type of tumor suppressor gene. The relationship between MnSOD activity and the malignant phenotype was studied by transfection of MnSOD cDNA into the SV40-transformed human fibroblast cell line WI-38 VA13 subline 2RA. The inte...
متن کاملAbsence of aminonucleoside-sensitive steps in the cell cycle of SV40-transformed human fibroblasts.
Puromycin aminonucleoside arrests cultured normal hu man lung fibroblasts in the G, and G2 phases of the cell cycle but permits fibroblasts transformed by the oncogenic virus SV40 to continue their cell-cycle traverse. Amino nucleoside has no detectable effect on the entry of the transformed cells into mitosis but it does accelerate the rate of detachment of these cells from the growth surface....
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ژورنال
عنوان ژورنال: Carcinogenesis
سال: 1996
ISSN: 0143-3334,1460-2180
DOI: 10.1093/carcin/17.12.2589